tsaD [2019-05-24 09:02:14]

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein

locus: BSU05940

pI: 5.02

mw: 36.69 kDa

protein length: 346 aa

gene length: 1041 bp

function: tRNA modification

product: tRNA modification enzyme

essential: yes

ec: 2.3.1.234

synonyms: ydiE,gcp

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

Gene

Coordinates: 643,258 644,298

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE]) [Pubmed|23072323,21183954]

Modification

• in vitro phosphorylated by [protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE] [pubmed|28890133]

Structure

• [PDB|3ZET] (from ''Salmonella typhimurium'', 46% identity) [Pubmed|23471679]

Additional information

• [SW|universally conserved protein]

Expression and Regulation

Operons

• genes: [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|ydiD]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
  description: [Pubmed|22383849]

References

Publications on the corresponding protein from other species

• 21183954,19376873,20824107,21619589,21285948,21625506,23056478,23471679,28890133,29741707